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KMID : 0379119850130040213
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Korean Journal of Mycology
1985 Volume.13 No. 4 p.213 ~ p.219
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Studies on Cellulolytic Enzymes Produced by Pleurotus spp . in Synthetic Medium (1)
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김정숙/Kim, Jeong Sook
이종배/홍재식/김명곤/고무석/이극로/Lee, Jong Bae/Hong, Jai Sik/Kim, Myung Kon/Koh, Moo Seok/Lee, Keug Ro
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Abstract
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Among the eight strains, Pleurotus sajor-caju JAFM 1017 was selected as most potent producer of cellulolytic enzymes. The avicelase and CMCase activity reached maximum levels after 10 days, and β-glucosidase activity reached a maximum level after 19 days. Among the various carbon sources, cellulose powder was most effective for the production of avicelase and β-glucosidase, and Na-CMC (sodium carboxymethyl cellulose) was good for the production of CMCase. The optimum concentration of cellulose powder was 1.0% (w/v), and glucose (1.0%) completely depressed the production of enzymes. Nitrates were effective for the production of enzymes, but nitrites did not support growth. The production of cellulolytic enzymes increased as the concentration of urea increased. The appropriate concentration of urea was 0.054% (w/v).
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